- Title
- FtsK-dependent XerCD-dif recombination unlinks replication catenanes in a stepwise manner
- Creator
- Shimokawa, Koya; Ishihara, Kai; Grainge, Ian; Sherratt, David J.; Vazquez, Mariel
- Relation
- ARC.FT120100153 & NHMRC.1005697 http://purl.org/au-research/grants/arc/FT120100153
- Relation
- Proceedings of the Natlonal Academy of Sciences Vol. 110, Issue 52, p. 20906-20911
- Publisher Link
- http://dx.doi.org/10.1073/pnas.1308450110
- Publisher
- National Academy of Sciences
- Resource Type
- journal article
- Date
- 2013
- Description
- In Escherichia coli, complete unlinking of newly replicated sister chromosomes is required to ensure their proper segregation at cell division. Whereas replication links are removed primarily by topoisomerase IV, XerC/XerD-dif site-specific recombination can mediate sister chromosome unlinking in Topoisomerase IV-deficient cells. This reaction is activated at the division septum by the DNA translocase FtsK, which coordinates the last stages of chromosome segregation with cell division. It has been proposed that, after being activated by FtsK, XerC/XerD-dif recombination removes DNA links in a stepwise manner. Here, we provide a mathematically rigorous characterization of this topological mechanism of DNA unlinking. We show that stepwise unlinking is the only possible pathway that strictly reduces the complexity of the substrates at each step. Finally, we propose a topological mechanism for this unlinking reaction.
- Subject
- DNA topology; tangle method; Xer recombination; band surgery; topology simplification
- Identifier
- http://hdl.handle.net/1959.13/1339851
- Identifier
- uon:28354
- Identifier
- ISSN:1091-6490
- Language
- eng
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